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mouse igg2b kappa light chain isotype control mg2b  (Novus Biologicals)


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    Novus Biologicals mouse igg2b kappa light chain isotype control mg2b
    Mouse Igg2b Kappa Light Chain Isotype Control Mg2b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg2b+kappa+light+chain+isotype+control+mg2b/Mouse+IgG2b+Kappa+Light+Chain+Isotype+Control+(MG2b)/pm41771834-99-186-168
    Average 94 stars, based on 3 article reviews
    mouse igg2b kappa light chain isotype control mg2b - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells
    Article Snippet: To analyse protein expression, following digestion and centrifugation, hNSCs and hOPCs were resuspended in TF Perm/Wash buffer (Cat.#0114775, BD Biosciences, NJ, USA) centrifuged at 2000 × g for 5 min. Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26 °C. hNSCs ( n = 3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti-human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs ( n = 3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti-human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti-human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26 °C.

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells.
    Article Snippet: Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26°C. hN Cs (n =3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs (n =3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26°C.

    Suspension:

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells
    Article Snippet: To analyse protein expression, following digestion and centrifugation, hNSCs and hOPCs were resuspended in TF Perm/Wash buffer (Cat.#0114775, BD Biosciences, NJ, USA) centrifuged at 2000 × g for 5 min. Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26 °C. hNSCs ( n = 3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti-human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs ( n = 3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti-human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti-human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26 °C.

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells.
    Article Snippet: Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26°C. hN Cs (n =3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs (n =3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26°C.

    Incubation:

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells
    Article Snippet: To analyse protein expression, following digestion and centrifugation, hNSCs and hOPCs were resuspended in TF Perm/Wash buffer (Cat.#0114775, BD Biosciences, NJ, USA) centrifuged at 2000 × g for 5 min. Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26 °C. hNSCs ( n = 3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti-human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs ( n = 3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti-human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti-human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26 °C.

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells.
    Article Snippet: Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26°C. hN Cs (n =3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs (n =3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26°C.

    Staining:

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells
    Article Snippet: To analyse protein expression, following digestion and centrifugation, hNSCs and hOPCs were resuspended in TF Perm/Wash buffer (Cat.#0114775, BD Biosciences, NJ, USA) centrifuged at 2000 × g for 5 min. Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26 °C. hNSCs ( n = 3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti-human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs ( n = 3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti-human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti-human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26 °C.

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells.
    Article Snippet: Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26°C. hN Cs (n =3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs (n =3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26°C.

    Control:

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells
    Article Snippet: To analyse protein expression, following digestion and centrifugation, hNSCs and hOPCs were resuspended in TF Perm/Wash buffer (Cat.#0114775, BD Biosciences, NJ, USA) centrifuged at 2000 × g for 5 min. Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26 °C. hNSCs ( n = 3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti-human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs ( n = 3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti-human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti-human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26 °C.

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells.
    Article Snippet: Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26°C. hN Cs (n =3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs (n =3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26°C.

    Expressing:

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells
    Article Snippet: To analyse protein expression, following digestion and centrifugation, hNSCs and hOPCs were resuspended in TF Perm/Wash buffer (Cat.#0114775, BD Biosciences, NJ, USA) centrifuged at 2000 × g for 5 min. Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26 °C. hNSCs ( n = 3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti-human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs ( n = 3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti-human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti-human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26 °C.

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells.
    Article Snippet: Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26°C. hN Cs (n =3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs (n =3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26°C.

    Centrifugation:

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells
    Article Snippet: To analyse protein expression, following digestion and centrifugation, hNSCs and hOPCs were resuspended in TF Perm/Wash buffer (Cat.#0114775, BD Biosciences, NJ, USA) centrifuged at 2000 × g for 5 min. Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26 °C. hNSCs ( n = 3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti-human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs ( n = 3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti-human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti-human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26 °C.

    Article Title: Multi-omics reveals heterogeneity and functional populations of oligodendrocyte progenitor cells induced by human neural stem cells.
    Article Snippet: Human TruStain Fc XTMFc Receptor Blocking Solution (5 μL/test, Cat. #422302, Biolegend) was added to the cell suspension and incubated for 10 minutes at 26°C. hN Cs (n =3, from three different biological replicates) were stained with PE Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.# 554680, BD Biosciences), PE Nestin mouse-anti human (5 μL/Test, Cat.#561230, BD Biosciences), Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#557721, BD Biosciences), Alexa Fluor®AF488 Vimentin mouse anti-human (8 μL/Test, Cat.#562338, BD Biosciences), PE Mouse IgG1 κ Isotype Control, PE Musashi mouse anti-human (5 μL/Test, Cat.#561468, BD Biosciences), Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (5 μL/Test, Cat.#566011, BD Biosciences) and Alexa Fluor® 647 PAX6 mouse anti-human (5 μL/Test, Cat.#562249, BD Biosciences), respectively; hOPCs (n =3) were stained with BV421 Mouse IgG2a, k Isotype Control (5 μL/Test, Cat.# 562439, BD Biosciences), BV421 PDGFR-α mouse-anti human (5 μL/Test,Cat.#562799,BD Biosciences), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] (5 μL/Test, Cat.#NBP1-43954, NOVUS Biologicals), FITC SOX10 mouse-anti human (10 μL/Test, Cat.#NBP2-47709F, NOVUS Biologicals), PE Mouse IgG1, κ Isotype Control, PE A2B5 mouse anti human (10 μL/Test, Cat.#130-093-581, Miltenyi Biotec), Mouse IgG2b Kappa Light Chain Isotype Control (MG2b) [FITC] and FITC OLIG2 mouse-anti human (10 μL/Test, Cat.#AB9610, Millipore) antibodies, respectively incubated for 30 minutes at 26°C.



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    Novus Biologicals mouse igg2b kappa light chain isotype control mg2b
    Mouse Igg2b Kappa Light Chain Isotype Control Mg2b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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